KCI BioTech (Suzhou) Inc.
KCI·KMQ has established an anti infection research and service platform with dual qualifications of BSL2 and ABSL2. The BSL-2 laboratory covers an area of approximately 200m ² and includes cells, viruses, and bacteria; The experimental area of ABSL2 is about 1000m ², including 300m ² for small animal laboratory and 700m ² for large animal laboratory. This platform is equipped with a professional operation and technical team, including senior scientists, dedicated laboratory personnel, dedicated veterinarians, and a QA management system. The staff have received special training in professional operations, obtained relevant qualifications, and carried out related work under the guidance and management of scientists with experience in anti infection research and development.
After nearly 2 years of internal research and development and external services, this platform has completed the filing of more than 70 pathogenic microorganisms, successfully established more than 20 animal models of viral and bacterial fungal infections, and has cooperated with more than 20 new drug research and development enterprises in the fields of human vaccines and anti infective drugs, animal vaccines and anti infective drugs, etc., to assist in the development and application of preclinical drugs. At present, the main businesses involved include:
Human vaccines and anti infective drugs, veterinary vaccines and anti infective drugs(1)In vitro anti infective efficacy test:
Antiviral activity - EC50/CC50 detection; MIC drug sensitivity test; FIC measurement; Effects of biological characteristics, etc.(2)Immunogenicity testing of vaccines in vivo: neutralization test, hemagglutination inhibition test, cellular immunity test, etc;
(3)Animal protective testing against viruses: vaccines, antiviral drugs;
(4)Infectious animal testing.List of Animal Model Information for KCI KMQ Biological Anti Infection Service | |||||||||||
classification | name | subtype | model | Animal species | |||||||
mice | rat | cotton mouse | guinea pig | pig | cat | ferret | rabbit | ||||
virus | respiratory syncytial virus | A2/ 9320/ 18537 | Attack on Poison Model | √ |
| √ |
|
|
|
|
|
HBV |
| AAV-HBV1.3 infection model | √ |
|
|
|
|
|
|
| |
flu virus | H1N1/H3N2 | Attack on Poison Model | √ |
|
|
|
|
| √ |
| |
Human herpesvirus 2 (HSV-2) | MS | Reproductive tract infection model | √ |
|
| √ |
|
|
|
| |
Herpes zoster virus (VZV) | Oka | Neuralgia/Skin Herpes |
| √ |
| √ |
|
|
|
| |
Feline infectious peritonitis virus | FCoV II | Cat transmitted abdominal infection model |
|
|
|
|
| √ |
|
| |
Rabbit Papilloma Skin Disease Model | NA | Rabbit CRPV virus skin papilloma |
|
|
|
|
|
|
| √ | |
Porcine Blue Ear Virus (PRRSV) | High fever type | Porcine Reproductive and Respiratory Syndrome Virus Infection Model |
|
|
|
| √ |
|
|
| |
bacteria | Staphylococcus aureus | Seattle 1945 | Skin wound infection model |
|
|
|
| √ |
|
|
|
Septicemia model | √ |
|
|
|
|
|
|
| |||
Escherichia Coli | Seattle 1946 | Septicemia model | √ |
|
|
|
|
|
|
| |
enterobacter cloacae | Clinical strain | Model of thigh muscle infection | √ |
|
|
|
|
|
|
| |
Acinetobacter baumannii | Clinical strain | Pneumonia model |
| √ |
|
|
|
|
|
| |
Model of thigh muscle infection | √ |
|
|
|
|
|
|
| |||
klebsiella pneumoniae | C122 | Infectious pneumonia model | √ |
|
|
|
|
|
|
| |
Pseudomonas aeruginosa | ATCC9027 | Infectious model of pneumonia | √ |
|
|
|
|
|
|
| |
Septicemia model | √ |
|
|
|
|
|
|
| |||
Model of thigh muscle infection | √ |
|
|
|
|
|
|
| |||
fungus | Candida albicans | 3147 | Vaginitis model |
| √ |
|
|
|
|
|
|
Septicemia model | √ |
|
|
|
|
|
|
| |||
Pneumonia model | √ |
|
|
|
|
|
|
experiment | Isolation and cultivation of bacteria/fungi/viruses | ||
In vitro antiviral CC50 and EC50 testing | |||
In vitro antimicrobial susceptibility test | Minimum inhibitory concentration (MIC) determination | 1. Micro broth dilution method | |
2. Paper amplification method | |||
FIC assay chessboard method combined with antibacterial ability determination | |||
Influence of biological characteristics | 1. Growth curve | ||
2. Sports force | |||
3. Biofilm | |||
4. Adhesive invasion | |||
Evaluation of vaccine immunogenicity | Humoral immune evaluation | 1. Combining IgG antibodies and detecting different subtypes | |
2.Neutralization test/hemagglutination inhibition test | |||
Cellular immune evaluation | ELISOPT IFN - γ/IL-2/IL-4/IL-6 detection | ||
Flow detection - CD4+\ CD8+\ IFN - γ+\ TNF - α+\ IL-2+ \ IL-4+\ IL-6+and other detection methods | |||
Anti toxic protective experiment | |||
Evaluation of therapeutic anti infective drugs |